Totally Spies features the adventures and missions of three teenage spies (Sam, Clover, and Alex) led by the founder and administrator of WOOHP, Jerry Lewis. The surnames of Clover and Alex is unknown or not revealed.
Samantha Simpsoms (or Sam for short) has long, wavy bright orange hair. She wears a green catsuit. She is the most intelligent, responsible, and mature of the girls. She always comforts the girls when they are in trouble. She also prevents the others from getting distracted. Her fav colors are purple and green.
Clover has medium-short, straight, blonde hair. She wears a red catsuit. Clover is a boy-crazy shopaholic and fashion fanatic. Mandy is her rival. She goes through many boys in the show, and she seems to care deeply about fashion and designer things. Her fav colors are blue and red.
Alexandra (or Alex for short) has short black hair, styled in a bob. She wears a bright yellow catsuit. Despite her clumsiness, Alex excels in athletics-- particularly in soccer. She is funny and always there for her friends. Though she is somewhat of a tomboy compared to Clover and Sam, Alex is just as eager when it comes to fashion and boys. She has a toy turtle named Ollie.Her fav colors are pink and yellow.
Jerry is the founder and administrator of W.O.O.H.P (World Organization Of Human Protection). He is a middle-aged, very serious British gentleman. He finds means of transporting the girls to their headquarters, briefs them on their missions, distributes their gadgets, and provides mission support with information or direct involvement. He often annoys the girls with his attitude and constant missions but when in danger the girls are there to help. It is also known that he has trouble keeping his spy identity a secret from his nosy mother, who has only been mentioned a few times in the season. Sam, Clover and Alex once noted that they don't listen to anyone but Jerry, and usually they don't even listen to him.